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  • Probe-based bacterial single-cell RNA sequencing predicts . . .
    Here we developed probe-based bacterial sequencing (ProBac-seq), a method that uses libraries of DNA probes and an existing commercial microfluidic platform to conduct bacterial single-cell RNA
  • Bacterial droplet-based single-cell RNA-seq reveals . . . - PubMed
    We introduce BacDrop, a highly scalable technology for bacterial single-cell RNA sequencing that has overcome many challenges hindering the development of scRNA-seq in bacteria BacDrop can be applied to thousands to millions of cells from both gram-negative and gram-positive species It features un …
  • Identification of intracellular bacteria from multiple single . . .
    One increasingly popular approach for studying intracellular or host cell–associated microbes is the analysis of microbial reads from single-cell RNA sequencing (scRNA-seq) in the settings of viral infection or of cancer (17 – 21)
  • Improved Bacterial Single-Cell RNA-Seq through Automated MATQ . . .
    ABSTRACT Bulk RNA sequencing technologies have provided invaluable insights into host and bacterial gene expression and associated regulatory networks Nevertheless, the majority of these approaches report average expression across cell populations, hiding the true underlying expression patterns that are often heterogeneous in nature Due to technical advances, single-cell transcriptomics in
  • High-throughput single-cell transcriptomics of bacteria using . . .
    Through four rounds of combinatorial barcoding, this low-cost and high-throughput single-cell RNA sequencing method enables transcriptomic analysis of individual bacterial cells and the detection
  • Bacterial droplet-based single-cell RNA-seq reveals . . .
    BacDrop is a droplet-based technology for single-cell RNA-seq in bacteria that can be scaled to millions of bacterial cells or hundreds of samples and was used to elucidate transcriptionally distinct bacterial subpopulations associated with varying phenotypic outcomes linked to antibiotic resistance and persistence
  • Microbial single-cell RNA sequencing by split-pool barcoding
    Single-cell RNA sequencing (scRNA-seq) has become an essential tool for characterizing gene expression in eukaryotes, but current methods are incompatible with bacteria Here, we introduce microSPLiT (microbial split-pool ligation transcriptomics), a high-throughput scRNA-seq method for Gram-negativ …
  • Bacterial single cell RNA-seq reveals antibiotic resistance . . .
    While eukaryotic cells have been tested and validated extensively on single cell sequencing platforms, prokaryotic cells (in this case, bacteria) don’t follow the eukaryotic norms For example, one crucial difference between mammalian and bacterial cells is in the composition and accessibility of their messenger RNA (mRNA) molecules, or transcripts These molecules carry the coding sequences
  • Ushering in a new era of single-cell transcriptomics in bacteria
    Abstract Transcriptome analysis of individual cells by single-cell RNA-seq (scRNA-seq) has become routine for eukaryotic tissues, even being applied to whole multicellular organisms In contrast, developing methods to read the transcriptome of single bacterial cells has proven more challenging, despite a general perception of bacteria as much simpler than eukaryotes Bacterial cells are harder
  • Bacterial single-cell RNA sequencing captures biofilm . . .
    Here, we present an optimized bacterial single-cell RNA sequencing method, BaSSSh-seq, to study Staphylococcus aureus diversity during biofilm growth and transcriptional adaptations following





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