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  • Glial Cell Communities Conspire to Drive Alzheimer’s Disease
    The amyloid cascade—plaques to tangles to cognitive decline—has become well established Less well understood is what role inflammation and glial changes play Now, by analyzing single-nuclei RNA-Seq data from more than 400 postmortem brains, scientists have homed in on specific glial subtypes
  • Amelioration of Tau and ApoE4-linked glial lipid . . . - ALZFORUM
    Here, Alexandra Litvinchuk from David Holtzman’s laboratory investigated the effect of the LXR agonist GW3965 as well as overexpression of ABCA1 on tau and apoE4-linked glial lipid accumulation and neurodegeneration
  • Sans Plexin-B1, Glial Net Tightens Around Plaques - ALZFORUM
    Plexin-B1 expression by astrocytes imposes cell spacing in glial nets Without Plexin-B1, glia huddle more closely together, and with plaques The result? Smaller plaques, protected synapses, spared memory In mice
  • New Role for Tau: Making Lipid Droplets in Glia? | ALZFORUM
    When they cranked up tau expression in glia throughout the fly brain, glial lipid droplets plummeted by more than 90 percent, and peroxidated lipids accumulated Similarly, in co-cultures of rat primary cells grown in a salt solution that provokes mild oxidative stress, overexpressing tau in astrocytes slashed production of lipid droplets
  • “iAssembloids” Probe Glial Roles in Neuron Survival
    Interestingly, they discovered that this axis is undetectable in 2D or even 3D neuron monoculture models, highlighting the need for more sophisticated models replicating physiological neural-glial networks Notably, they detected and validated that a voltage-gated calcium channel beta subunit plays a role in APOE4 astrocyte-iAssembloids
  • Engineered 3D Immuno-Glial-Neurovascular Human Brain Model | ALZFORUM
    Stanton AE, Bubnys A, Agbas E, James B, Park DS, Jiang A, Pinals RL, Truong N, Loon A, Staab C, Liu L, Cerit O, Wen H-L, Kellis M, Blanchard JW, Langer RS, Tsai L-H Engineered 3D Immuno-Glial-Neurovascular Human Brain Model 2023 Aug 17 10 1101 2023 08 15 553453 (version 1) bioRxiv
  • Don’t Name It: Glial States Confound Easy Labels - ALZFORUM
    DAM, HAM, WAM, LDAM, MGnD, ARM, A1 A2—got them all straight? The list of names for microglial and astrocyte profiles is growing, because these glial cells change in diverse ways in response to brain pathology As recent studies have begun to explore this variety of reactive states in depth, an
  • Glial AP1 is activated with aging and accelerated by . . . - ALZFORUM
    Byrns CN, Saikumar J, Bonini NM Glial AP1 is activated with aging and accelerated by traumatic brain injury Nat Aging 2021 Jul;1 (7):585-597 Epub 2021 Jul 8 PubMed
  • Primary Mixed Glial Cultures From Mouse Cerebral Cortex
    This protocol describes the preparation of primary glial cultures consisting of ~75% astrocytes and ~25% microglia The protocol specifies neonatal mouse cerebral cortex, but also works for late embryos, for rat, and for most CNS regions
  • Stirred by Tau, Neurons Amp Up Lipid Droplets in Glia
    Lipid droplets accumulate in microglia in mouse and cell models of amyloidosis but scientists are not sure why Evidence suggests that Aβ helps create the glut, but now scientists led by Lingyan Shi and Xu Chen at the University of California, San Diego, report that mutant tau lends a hand In the





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